Evaluation of The Inhibitory Activity of Aqueous and Alcoholic (Punica granatum) Pomegranate Peel Extract on Some Bacterial species Isolated from Patients’ beds at Al-Refai Teaching Hospital
Abstract
Background: Pomegranate (Punica granatum) peel contains many active compounds such as tannins, phenols, and terpenes. Pomegranate has been used since ancient times in folk medicine to treat various stomach and intestinal diseases. People have also used it to treat epidermal and skin diseases, as well as to strengthen hair follicles. Aim: Evaluation of the inhibitory activity of alcoholic and aqueous extracts of pomegranate peel (P. granatum) on some antibiotic-resistant bacterial species in the patients' beds of the teaching hospital in Al-Rafai city. Which helps in improving the health and nursing care of hospital patients. Methods: Pomegranate peels were dried, ground, and extracted using methanol and water solvent. Bacteria were isolated from the patients' beds and two methods were used to test the sensitivity of microbes to the extracts, the first method was using disks and the second was using the density counting method. Nutrient broth was inoculated with single colonies of the isolated bacteria individually for each, then the medium was inoculated at 37°C for 24 h. Results: According to the study's findings, as it gave the lowest concentration of 2.5 mg/ml(minimum inhibition concentration (MIC) of the aqueous extract of the following readings (1.022, 1.186 0.698) at a wave length (595 nn) for each of E.coli, Staph. aureus and Strep pyogens respectively. Conclusions: This study found the efficacy of pomegranate peel in the inhibition of bacteria increases with increasing concentrations of the aqueous extract. It can be used to sterilize beds in hospitals, which helps improve health and nursing care for hospital patients.
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Algelal, S. M. H., Arab, K. A. H., Mussa, M. H., Guda, M. A., & Hadi, S. J. (2024). Investigation use irradiation to treat soil contaminate with oil. International Journal of Applied Sciences and Technology, 6(1), 141-150. Available at: http://dx.doi.org/10.47832/2717-8234.18.10
Ali, A. T., Guda, M. A., Oraibi, A. I., Salih, I. K., Shather, A. H., Abd Ali, A. T., ... & Almashhadani, H. A. (2023). Fe 3 O 4 supported [Cu (ii)(met)(pro-H) 2] complex as a novel nanomagnetic catalytic system for room temperature C–O coupling reactions. RSC Advances, 13(32), 22538-22548. https://doi.org/10.1039/d3ra03509c
Alias, M. F. A., & Abd–Alsada, A. S. (2021, December). The influence of zinc oxide with carbon nanotube composite nanomaterials on antibacterial activity. In Journal of Physics: Conference Series (Vol. 2114, No. 1, p. 012089). IOP Publishing. http://dx.doi.org/10.1088/1742-6596/2114/1/012089
Asad, M., & Alhomoud, M. (2016). Proulcerogenic effect of water extract of Boswellia sacra oleo gum resin in rats. Pharmaceutical Biology, 54(2), 225-230. https://doi.org/10.3109/13880209.2015.1028553
Cai, Z., Chattopadhyay, N., Liu, W. J., Chan, C., Pignol, J. P., & Reilly, R. M. (2011). Optimized digital counting colonies of clonogenic assays using ImageJ software and customized macros: comparison with manual counting. International Journal of Radiation Biology, 87(11), 1135-1146. https://doi.org/10.3109/09553002.2011.622033
Chouhan, S., Sharma, K., & Guleria, S. (2017). Antimicrobial activity of some essential oils—present status and future perspectives. Medicines, 4(3). https://doi.org/10.3390/medicines4030058
Dakah, A., & Maarrouf, M. (2019). Antileishmanial and antibacterial activity of essential oils of medicinal plant Achillea santolina L. Online Journal of Biological Sciences, 19(1), 69-76. https://doi.org/10.3844/ojbsci.2019.69.76
Guda, M. A. (2023, December). Response of antioxidant in some plants to iron oxide nanoparticles. In AIP Conference Proceedings (Vol. 2977, No. 1). AIP Publishing. https://doi.org/10.1063/5.0182305
Guda, M. A., & Ammar Semysim, E. A. A. A. (2022). Response of a medicinal plant peganum harmala to iron oxide nanoparticles F3O4(Nps). Journal of Pharmaceutical Negative Results, 13. https://doi.org/10.47750/pnr.2022.13.S08.123
Guda, M.A., Mutlag, N.H., Tsear, A.A. (2020). The use of Atriplex nummularia plant as the hyperaccumulators of silver. AIP Conference Proceedings, 2020, 2290, 0027565. https://doi.org/10.1063/5.0027565
Hamad, M. O., Rahi, A. Y., & Guda, M. A. (2023). Study of Staphylococcus spp. isolated from Clinical Cases and determination of its multidrugresistant to antibiotics and phytosynthesized ZnO (NPs). Cardiometry, (26), 227-235. http://dx.doi.org/10.18137/cardiometry.2023.26.227235
Husayn, D. M., & Guda, M. A. (2023a, July). Effect of zinc oxide nanoparticles on biomarkers of chlorophyll and carotene in some wild plants. In AIP Conference Proceedings (Vol. 2787, No. 1). AIP Publishing. https://dx. doi:10.1063/5.0148197
Husayn, D. M., & Guda, M. A. (2023b, July). Response of some wild plants in antioxidant enzymes by zinc oxide nanoparticles. In AIP Conference Proceedings (Vol. 2787, No. 1). AIP Publishing. http://dx.doi.org/10.1063/5.0148199
Kafil, T. S., Nguyen, T. M., Patton, P. H., MacDonald, J. K., Chande, N., & McDonald, J. W. (2017). Interventions for treating collagenous colitis. The Cochrane Database of Systematic Reviews, 11(11). https://doi.org/10.1002/14651858.CD003575.pub6
Kumaresan, M., Anand, K. V., Govindaraju, K., Tamilselvan, S., & Kumar, V. G. (2018). Seaweed Sargassum wightii mediated preparation of zirconia (ZrO2) nanoparticles and their antibacterial activity against gram positive and gram negative bacteria. Microbial pathogenesis, 124, 311-315. https://doi.org/10.1016/j.micpath.2018.08.060
Parthipan, P., AlSalhi, M. S., Devanesan, S., & Rajasekar, A. (2021). Evaluation of Syzygium aromaticum aqueous extract as an eco-friendly inhibitor for microbiologically influenced corrosion of carbon steel in oil reservoir environment. Bioprocess and Biosystems Engineering, 44, 1441-1452. https://doi.org/10.1007/s00449-021-02524-8
Qazmooz, H. A., Guda, M. A., Algburi, J. B., Al-Zurfi, S. K., & Al-Graiti, T. A. (2020). Determination of heavy metal in samples of Tirmania nivea fungi in different soils. Plant Archives, 20(2), 313-317. Available at: https://www.plantarchives.org/SPL%20ISSUE%2020-2/50__313-317.pdf
Rahi, A. Y., Guda, M. A., Abdulbary, M., & Khashan, K. T. (2009, August). Effect Biosynthesis of fenugreek leaves nanomaterial on some plant’s germination using saline water. Revis Bionatura 2023; 8(2) 60. chemical research. http://dx.doi.org/10.21931/RB/2023.08.02.60
Sabra, S. M., & Al-Masoudi, L. M. (2014). The effect of using frankincense (Boswellia sacra) chewing gum on the microbial contents of buccal/oral cavity, Taif, KSA. J. Dent. Med. Sci, 13(4), 77-82. http://dx.doi.org/10.9790/0853-13417782
Seil, J. T., & Webster, T. J. (2012). Antimicrobial applications of nanotechnology: methods and literature. International Journal of Nanomedicine, 2767-2781. https://doi.org/10.2147/ijn.s24805
Silness, J., & Löe, H. (1964). Periodontal disease in pregnancy II. Correlation between oral hygiene and periodontal condition. Acta Odontologica Scandinavica, 22(1), 121-135. https://doi.org/10.3109/00016356408993968
World Health Organization. (2004). Laboratory biosafety manual (4th ed.). World Health Organization. https://www.who.int/publications/i/item/9789240011311
Yedurkar, S., Maurya, C., & Mahanwar, P. (2016). Biosynthesis of zinc oxide nanoparticles using ixora coccinea leaf extract—a green approach. Open Journal of Synthesis Theory and Applications, 5(1), 1-14. http://dx.doi.org/10.4236/ojsta.2016.51001
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