Effect of Solvent Polarity on the Nutritional and Phytochemical Composition of Cocos nucifera Bee Pollen

Abstract

Background: Monofloral bee pollen is increasingly recognized as a nutritionally valuable natural product with promising applications in functional foods and herbal medicine. The present study evaluated the physicochemical, phytochemical, and nutritional characteristics of Cocos nucifera bee pollen extracted using solvents of varying polarity, namely acetone, ethanol, methanol, aqueous, and hydroethanolic solvents. Methods: Cocos nucifera bee pollen (CNBP) pellets were extracted by cold maceration, and the obtained extracts were subjected to physicochemical analysis, qualitative phytochemical screening, and quantitative nutritional evaluation using standard biochemical methods. Results: Extraction yield varied significantly with solvent polarity, with methanol extract exhibiting the highest yield (38%), indicating efficient recovery of polar bioactive constituents. Physicochemical parameters confirmed the stability and quality of the extracts. Qualitative phytochemical analysis revealed the presence of alkaloids, flavonoids, phenols, tannins, saponins, steroids, glycosides, and proteins in different solvent extracts. Quantitative analysis demonstrated that CNBPis a rich source of carbohydrates, proteins, amino acids, dietary fibre, vitamins, lipids, cardiac glycosides, and essential minerals. Aqueous extracts exhibited higher protein (159.65 ± 0.41 mg BSA/g) and flavonoid contents (366.6 ± 0.57 mg QE/g), whereas methanolic extracts showed elevated phenols and alkaloids. Hydroethanolic extracts demonstrated higher amino acid concentrations, suggesting improved extraction efficiency for mixed-polarity biomolecules. Conclusion: The findings indicate that solvent polarity strongly influences the extraction efficiency and phytochemical composition of CNBP. Overall, the study highlights the nutraceutical potential and supports its prospective application in functional food and herbal formulations. Further pharmacological and clinical investigations are warranted to validate its therapeutic potential.

Keywords: Cocos Nucifera Bee Pollen, Functional Foods, Phytochemicals, Nutritional Profiling

Downloads

Download data is not yet available.

References

Abner, A. (2025). ron Deficiency and Athletics Performance: Implication, Interventions, and Strategies for Optimization. JSU Student Symposium, 77. https://digitalcommons.jsu.edu/ce_jsustudentsymp_2025/77
Aborisade, A. (2017). Phytochemical and proximate analysis of some medicinal leaves. Clinical Medicine Research. https://doi.org/10.11648/j.cmr.20170606.16
Achi, N. K., Onyeabo, C., Ekeleme-Egedigwe, C. A., &Onyeanula, J. C. (2017). Phytochemical, proximate analysis, vitamin and mineral composition of aqueous extract of Ficus capensis leaves in South Eastern Nigeria. Journal of Applied Pharmaceutical Science, 7(3), 117-122. https://dx.doi.org/10.7324/JAPS.2017.70319
Ahmed, M., Marrez, D. A., Abdelmoeen, N. M., Mahmoud, E. A., Abdel-Shakur Ali, M., Decsi, K., & Tóth, Z. (2023). Proximate analysis of Moringa oleifera leaves and the antimicrobial activities of successive leaf ethanolic and aqueous extracts compared with green chemically synthesized Ag-NPs and crude aqueous extract against some pathogens. International Journal of Molecular Sciences, 24(4), 3529. https://doi.org/10.3390/ijms24043529
Alghamdi, M., Gutierrez, J., & Komarnytsky, S. (2022). Essential minerals and metabolic adaptation of immune cells. Nutrients, 15(1), 123. https://doi.org/10.3390/nu15010123
Aylanc, V., Falcao, S. I., & Vilas-Boas, M. (2023). Bee pollen and bee bread nutritional potential: Chemical composition and macronutrient digestibility under in vitro gastrointestinal system. Food Chemistry, 413, 135597. https://doi.org/10.1016/j.foodchem.2023.135597
Bakır, A., Ekin, S., & Fırat, M. (2025). First report on determination of bioelement, vitamin content and antioxidant properties of Verbascum golawanense. International Journal of Secondary Metabolite, 12(3), 548-560. https://doi.org/10.21448/ijsm.1584793
Balabanova, L., Averianova, L., Marchenok, M., Son, O., &Tekutyeva, L. (2021). Microbial and genetic resources for cobalamin (vitamin B12) biosynthesis: From ecosystems to industrial biotechnology. International Journal of Molecular Sciences, 22(9), 4522. https://doi.org/10.3390/ijms22094522
Bertoncelj, J., Lilek, N., & Korošec, M. (2024). Bee pollen carbohydrates composition and functionality. In Pollen chemistry & biotechnology (pp. 51-69). Cham: Springer International Publishing. https://doi.org/10.1007/978-3-031-47563-4_3
Bhattacharjee, S., Sultana, A., Sazzad, M. H., Islam, M. A., Ahtashom, M., &Asaduzzaman, M. (2013). Analysis of the proximate composition and energy values of two varieties of onion (Allium cepa L.) bulbs of different origin: A comparative study. International Journal of Nutrition and Food Sciences, 2(5), 246-253. https://doi.org/10.11648/j.ijnfs.20130205.16
Blachier, F. (2025). Characteristics of the Different Amino Acids Found in the Living World. In: The Evolutionary Journey of Amino Acids. Fascinating Life Sciences. Springer, Cham. https://doi.org/10.1007/978-3-031-93393-6_1
Bobiş, O., Urcan, A. C., De-Melo, A. A. M., & de Almeida-Muradian, L. B. (2025). Chemical composition of bee pollen. In Bee Products–Chemical and Biological Properties (pp. 339-390). Cham: Springer Nature Switzerland.https://doi.org/10.1007/978-3-031-89049-9_11
Boni, C. B., Coppola, F., Sagona, S., Quaranta, M., Flaminio, S., Biella, P., ... &Felicioli, A. (2025). Pollen Resource Repartition Between Managed Honey Bees (Apis mellifera L. 1758) and Unmanaged Bees in Three Italian National Parks. Conservation, 5(1), 5. https://doi.org/10.3390/conservation5010005
Borpatra, P. J., & Saikia, B. S. (2025). Zinc Supplementation as a Preventive Measure for Zinc Deficiency. In Strategies to Alleviate Human Zinc Deficiency (pp. 159-192). IGI Global Scientific Publishing. https://doi.org/10.4018/979-8-3693-5355-4.ch007
Cavallero, A., Vidotto, F., Sbrana, C., Peres Fabbri, L., Petroni, G., & Gabriele, M. (2025). Antioxidant-rich polyfloral bee pollen exerts antimicrobial activity and anti-inflammatory effect in A549 lung epithelial cells by modulating the NF-κB pathway. Foods, 14(5), 802. https://doi.org/10.3390/foods14050802
Choi, S. R., & Lee, J. M. (2025). Study on the valorization of oak bark biomass-Part I: Extraction and characterization of tannins. Journal of Korea TAPPI, 57(6), 66-74. https://doi.org/10.7584/JKTAPPI.2025.12.57.6.66
Colzani, J., de Borba Júnior, J. C., Masote, J. B. B., Schiquet, S., Wayszceyk, S., Hochheim, S., ... & de Cordova, C. M. M. (2025). Metabolic effects of a low-dose propolis extract from Apis mellifera on the glycemic and lipid profile, insulin resistance, and markers of oxidative stress in a randomized double-blind clinical trial. Pharmacological Research-Natural Products, 100329. https://doi.org/10.1016/j.prenap.2025.100329
Dagher, W., Alassod, A., Al Hinnawi, M. F., Alghoraibi, I., Taher, A., &Alnhlaoui, M. (2025). Characterization and antibacterial effect of green-synthesised silver nanoparticles using different extraction methods from Ziziphus spina-christi (Sidr) leaf extract collected from Syria. RSC advances, 15(42), 35642-35659. https://doi.org/10.1039/D5RA05214A
De-Melo, A. A. M., & de Almeida-Muradian, L. B. (2017). Chemical Composition of Bee Pollen. In: Alvarez-Suarez, J. (eds) Bee Products - Chemical and Biological Properties. Springer, Cham. https://doi.org/10.1007/978-3-319-59689-1_11
Deutz, N. E., Wolfe, R. R., & Engelen, M. P. (2025). A new concept to establish protein requirements. Clinical Nutrition, 48, 1-5.https://doi.org/10.1016/j.clnu.2025.02.002
Dos Santos, C. P. C., Cruel, P. T. E., Buchaim, D. V., da Cunha, M. R., Ervolino, E., Issa, J. P. M., ... &Buchaim, R. L. (2025). Calcium hydroxyapatite combined with photobiomodulation for bone tissue repair: a systematic review. Materials, 18(5), 1120. https://doi.org/10.3390/ma18051120
El-Seedi, H. R., Eid, N., Abd El-Wahed, A. A., Rateb, M. E., Afifi, H. S., Algethami, A. F., ... & Khalifa, S. A. (2022). Honey bee products: preclinical and clinical studies of their anti-inflammatory and immunomodulatory properties. Frontiers in Nutrition, 8, 761267. https://doi.org/10.3389/fnut.2021.761267
Embark, J. A. (2025). Impact of Bee Pollen Supplementation on Growth Performance, Blood Parameters, Antioxidant Status, And Immune Response in Quail. African Journal of Advanced Pure and Applied Sciences, 4(1), 310-318. https://doi.org/10.65418/ajapas.v4i1.1160
Feás, X., Vázquez-Tato, M. P., Seijas, J. A., Pratima G. Nikalje, A., & Fraga-López, F. (2020). Extraction and physicochemical characterization of chitin derived from the Asian hornet, Vespa velutinaLepeletier 1836 (Hym.: Vespidae). Molecules, 25(2), 384. https://doi.org/10.3390/molecules25020384
Fioramonti, X., Pénicaud, L., Fioramonti, X., &Pénicaud, L. (2019). Carbohydrates and the brain: roles and impact. Feed your mind-How does nutrition modulate brain function throughout life, 386-390. https://books.google.co.in/books?hl=en&lr=&id=DhT8DwAAQBAJ&oi=fnd&pg=PA39&dq=Fioramonti,+X.,+P%C3%A9nicaud,+L.,+Fioramonti,+X.,+%26+P%C3%A9nicaud,+L.+(2019).+Carbohydrates+and+the+brain:+roles+and+impact.+Feed+your+mind-How+does+nutrition+modulate+brain+function+throughout+life,+386-390.&ots=2YsLLV1KWC&sig=9T4DKDc7QM0LW_3V6HpNh9d31Is&redir_esc=y#v=onepage&q&f=false
Fu, Y., Hou, L., Han, K., Zhao, C., Hu, H., & Yin, S. (2025). The physiological role of copper: Dietary sources, metabolic regulation, and safety concerns. Clinical Nutrition, 48, 161-179. https://doi.org/10.1016/j.clnu.2025.03.023
Galanakis, C. M., Goulas, V., Tsakona, S., Manganaris, G. A., & Gekas, V. (2013). A knowledge base for the recovery of natural phenols with different solvents. International Journal of Food Properties, 16(2), 382-396.https://doi.org/10.1080/10942912.2010.522750
Guo, B., Yi, X., Sun, Q., Sun, K., Guo, L., & Guo, Y. (2026). The Honey Bee Body Surface as a Microbial Hub: Connectivity Shaped by Monoculture vs. Polyculture Farming. Insects, 17(1), 53. https://doi.org/10.3390/insects17010053
Hait, M., Kashyap, N. K., Chandel, S. S., & Vaishnav, M. M. (2023). Proximate Analysis of Herbal Drugs: Methods, Relevance, and Quality Control Aspects. In: Izah, S.C., Ogwu, M.C., Akram, M. (eds) Herbal Medicine Phytochemistry. Reference Series in Phytochemistry. Springer, Cham. https://doi.org/10.1007/978-3-031-21973-3_42-1
Halimatushadyah, E., Sailah, I., Tallei, T. E., Tamala, Y., Safitri, L., &Humaedi, A. (2025). In vitro and In silico study of antioxidants and anti-inflammatory activity of bitter leaves (Vernonia amygdalina del.) Extract. Tropical Journal of Natural Product Research, 9(2), p. 534. DOI: https://doi.org/10.26538/tjnpr/v9i2.17
Ibrahim, S. K., Albadr, R. J., Sur, D., Yadav, A., Menon, S. V., Shit, D., ... & Mushtaq, H. (2025). Prediction of thermodynamic properties of aqueous carbohydrates solution using the PHSC and ANN models. Scientific Reports, 15(1), 21539. https://doi.org/10.1038/s41598-025-06552-2
Javed, A., Hashmi, M. S., Javaid, U., & Amjad, R. (2025). Classification and Therapeutic Applications of Plant Secondary Metabolites. Pharmacognosy and Phytochemistry: Principles, Techniques, and Clinical Applications, 189-206. https://doi.org/10.1002/9781394203680.ch10
Kalaskar, M., Yele, S. U., Ayyanar, M., Gurav, N., Beldar, V., & Surana, S. J. (2025). Methods of extraction. Pharmacognosy and Phytochemistry: Principles, Techniques, and Clinical Applications, 121-142. https://doi.org/10.1002/9781394203680.ch07
Lau, P., Lesne, P., Grebenok, R. J., Rangel, J., & Behmer, S. T. (2022). Assessing pollen nutrient content. Philosophical Transactions: Biological Sciences, 377(1853), 1-14. https://doi.org/10.1098/rstb.2021.0510
Leja, M., Mareczek, A., Wyżgolik, G., Klepacz-Baniak, J., &Czekońska, K. (2007). Antioxidative properties of bee pollen in selected plant species. Food Chemistry, 100(1), 237-240. https://doi.org/10.1016/j.foodchem.2005.09.047
Lenny, S., Surbakti, F. B., &Tarigan, J. B. (2025). Phytochemical Screening, Determination of Total Phenolic Contents and Total Flavonoid Contents of Pod Purified Extract from Caesalpinia pulcherrima (L.) Sw. Journal of Chemical Natural Resources, 7(1), 10-19. https://doi.org/10.32734/jcnar.v7i1.19838
Majumdar, A., Saraf, S. K., Sahu, C., Pathak, P., & Baghel, M. (2025). Zinc deficiency: Impact on growth, immunity, and the role of plant‐based nutritional strategies. Chemistry & Biodiversity, 22(10), e00325. https://doi.org/10.1002/cbdv.202500325
Maurya, N. K., Kushwaha, N., Keservani, R. K., Arya, P., & Yadav, R. (2025). Nutraceutical potential of bee pollen and a natural approach to health and wellness: a comprehensive review. Journal of Experimental Zoology India, 28(1), 77-85. https://doi.org/10.51470/jez.2025.28.1.77
Mensah, C., Bavoh, C. B., & Otu-Koi, A. A. (2025). Amino Acids Fundamentals. In Amino Acids Applications in the Geoenergy Industry (pp. 7-24). CRC Press. https://doi.org/10.1201/9781003502388-2
Mohamed, E. T., Mahmood, F. N., Merie, G. M. S., Saadi, A. M., Parvez, S., Fatima, G., ... & Gupta, G. (2025). The medical importance of sodium and potassium. International Journal of Pharma Growth Research Review, 2(2), 01-10. https://doi.org/10.54660/IJPGRR.2025.2.2.01-10
Nandhini, V. M., Priyadharshini, K. M., Thangapandiyan, S., & Thirumalai, V. (2026). Phytochemical Profiling and Cardiometabolic Potential of Cocos nucifera Bee Pollen: Antioxidant Insights from FTIR, LC-MS and GC-MS Analyses. Current Trends in Biotechnology & Pharmacy, 20(1), 2774. https://doi.org/10.5530/ctbp.2026.1.6
Nawaz, H., Shad, M. A., Rehman, N., Andaleeb, H., & Ullah, N. (2020). Effect of solvent polarity on extraction yield and antioxidant properties of phytochemicals from bean (Phaseolus vulgaris) seeds. Brazilian Journal of Pharmaceutical Sciences, 56, e17129. https://doi.org/10.1590/s2175-97902019000417129
Omimakinde, E. A. (2024). Assessment of Nutritional Compositions and Valuable Mineral Content of Some Nigerian Coconut Varieties.Journal of Biotechnology and Food Engineering, 2(1). https://doi.org/10.5281/zenodo.12724187
Omole, J. G., Udom, G. J., Aturamu, A., Agbana, R. D., Aziakpono, O. M., Oritsemuelebi, B., ... &Yemitan, O. K. (2025). Cardiac glycosides: Looking beyond heart failure and atrial fibrillation. Indian Journal of Pharmacology, 57(1), 33-47. https://doi.org/10.4103/ijp.ijp_934_24
Patra, A. K. (2012). An overview of antimicrobial properties of different classes of phytochemicals. Dietary Phytochemicals and Microbes, 1-32. https://doi.org/10.1007/978-94-007-3926-0_1
Razzaque, M. S., &Wimalawansa, S. J. (2025). Minerals and human health: From deficiency to toxicity. Nutrients, 17(3), 454. https://doi.org/10.3390/nu17030454
Sandhu, S., Naz, S., Ejidike, I. P., & Ata, A. (2025). Carbohydrates: Classification and Biosynthesis. In Synthesis and Applications of Carbohydrates, Lipids, and Steroids (pp. 333-342). CRC Press. https://doi.org/10.1201/9781003437413
Sanyal, A., Ghosh, A., Roy, C., Mazumder, I., & Marrazzo, P. (2023). Revolutionizing the use of honeybee products in healthcare: a focused review on using bee pollen as a potential adjunct material for biomaterial functionalization. Journal of Functional Biomaterials, 14(7), 352. https://doi.org/10.3390/jfb14070352
Shaikh, J. R., & Patil, M. (2020). Qualitative tests for preliminary phytochemical screening: An overview. International Journal of Chemical Studies, 8(2), 603-608. https://doi.org/10.22271/chemi.2020.v8.i2i.8834
Singh, P., Yadav, B., Kollur, S. P., & Singh, P. (2025). The Golden Superfruit: Unveiling the Nutritional Riches and Multifaceted Health Benefits of Seabuckthorn. Defence Life Science Journal, 10(4), 313-324. https://doi.org/10.14429/dlsj.21472
Singh, Y., Nimoriya, R., Rawat, P., Mishra, D. K., & Kanojiya, S. (2022). Quantitative evaluation of cardiac glycosides and their seasonal variation analysis in Nerium oleander using UHPLC‐ESI‐MS/MS. Phytochemical Analysis, 33(5), 746-753. https://doi.org/10.1002/pca.3126
Smith, M. J., Hoffman, N. J., San Jose, A. J., Burke, L. M., &Opar, D. A. (2025). Nutritional interventions to attenuate quadriceps muscle deficits following anterior cruciate ligament injury and reconstruction. Sports Medicine (Auckland, NZ), 55(3), 569. https://doi.org/10.1007/s40279-025-02174-w
Stefanache, A., Lungu, I. I., Butnariu, I. A., Calin, G., Gutu, C., Marcu, C., ... & Damir, D. (2023). Understanding how minerals contribute to optimal immune function. Journal of Immunology Research, 2023(1), 3355733. https://doi.org/10.1155/2023/3355733
Sun, S., Yu, Y., Jo, Y., Han, J. H., Xue, Y., Cho, M., ... & Zhuang, S. (2025). Impact of extraction techniques on phytochemical composition and bioactivity of natural product mixtures. Frontiers in Pharmacology, 16, 1615338. https://doi.org/10.3389/fphar.2025.1615338
Sun, Y., Xiong, F., Chen, Z., Wang, Z., Lu, X., Meng, X., ... & Zhou, Y. (2025). Enzymatically extracted rose bee pollen significantly alleviates BPH and hyperlipidemia-aggravated progression by targeting COX-2 inhibition. Food Bioscience, 107490.https://doi.org/10.1016/j.fbio.2025.107490
Verellen, F., Palottini, F., Estravis-Barcala, M. C., & Farina, W. M. (2025). Sugar conditioning combined with nectar nonsugar compounds enhances honey bee pollen foraging in a nectarlessdiocious crop. Scientific Reports, 15(1), 1756. https://doi.org/10.1038/s41598-025-85494-1
Vershinina, Y. S., Mitin, I. V., Garmay, A. V., Sugakov, G. K., & Veselova, I. A. (2025). Simple and robust approach for determination of total protein content in plant samples. Foods, 14(3), 358. https://doi.org/10.3390/foods14030358
Wang, L., Wang, L., Liu, X., Lin, X., Fei, T., & Zhang, W. (2025). Seaweeds-derived proteins and peptides: preparation, virtual screening, health-promoting effects, and industry applications. Critical reviews in food science and nutrition, 65(30), 6709-6736. https://doi.org/10.1080/10408398.2025.2449596
Wu, W., Gong, M., Liu, P., Yu, H., Gao, X., & Zhao, X. (2025). Hypomagnesemia: exploring its multifaceted health impacts and associations with blood pressure regulation and metabolic syndrome. Diabetology & Metabolic Syndrome, 17(1), 217. https://doi.org/10.1186/s13098-025-01772-y
Yaneva, Z., Grozeva, N., Todorova, M., Kamenova-Nacheva, M., Staleva, P., Memdueva, N., & Tzanova, M. T. (2025). Comparison of the Potential of “Green” Classical and Natural Deep Eutectic Solvents in the Production of Natural Food Colorant Extracts from the Roots of Alkanna tinctoria (L.). Foods, 14(4), 584. https://doi.org/10.3390/foods14040584
Yatoo, M. I., Gopalakrishnan, A., Saxena, A., Parray, O. R., Tufani, N. A., Chakraborty, S., ... & Iqbal, H. M. (2018). Anti-inflammatory drugs and herbs with special emphasis on herbal medicines for countering inflammatory diseases and disorders-a review. Recent Patents on Inflammation & Allergy Drug Discovery, 12(1), 39-58. https://doi.org/10.2174/1872213X12666180115153635
Yurdakok-Dikmen, B., & Filazi, A. (2025). Cardiovascular toxicity. In Veterinary Toxicology (pp. 197-209). Academic Press. https://doi.org/10.1016/B978-0-443-29007-7.00007-8
Statistics
16 Views | 1 Downloads
How to Cite
Manickam, N. and Marthandan, P. (2026) “Effect of Solvent Polarity on the Nutritional and Phytochemical Composition of Cocos nucifera Bee Pollen”, International Journal of Advancement in Life Sciences Research, 9(3), pp. 76-92. doi: https://doi.org/10.31632/ijalsr.2026.v09i03.007.